Hi Jim,
Yes, an immuno-electroporesis looking for heavy and light chains is
certainly cheaper.
Paula
At 05:29 PM 12/3/2001 -0500, Timothy Singleton, M.D. wrote:
>I have mixed feelings about performing flow cytometry for monoclonal
>gammopathies.
>These patients all have monoclonal plasma cells. Phenotyping just
>documents whether the
>clone is detectable by flow cytometry and whether there might be a
>phenotypic aberrancy
>that correlates with malignancy. Flow cytometry might not be necessary
>for diagnosis.
>
> From a patient care perspective multiple myeloma is incurable, except
> possibly for newer
>modalities, such as allogeneic bone marrow transplant. Some clinical
>textbooks just
>recommend following the patients and waiting for clinical signs of disease
>progression
>(anemia, lytic bone lesions, etc.) to decide when to initiate treatment.
>
>Tim Singleton, MD
>Royal Oak, MI
>
> >>> "Andrea Illingworth" <dcdsflow@mint.net> 11/30/01 04:03PM >>>
>Dear group,
>What are your thoughts on the use of CD40 to differentiate normal plasma
>cells from
>myeloma cells?
>Currently we are using the CD45/CD56/CD38 combination but we are looking
>into either
>adding CD138 and/or CD40. Would you recommend one over the other or
>recommend adding
>both antibodies?
>
>Thank you for your input!
>
>Andrea Illingworth
>Dahl-Chase Flow Cytometry
>Bangor, Maine 04401
>Andrea_Illingworth@dahlchase.com
>
Paula Fukushima
Flow Cytometry
LP, NCI, NIH
10 Center Drive MSC-1500
Bethesda, Md. 20892-1500
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