We use CD45 and CD38 both as gating parameters in every tube. This way we can gate on the CD45 neg/dim and CD38 bright population and then determine the CD19, CD56, CD138 and cytoplasmic light chain expression on those cells. Avis Peters United Hospital Flow Cytometry qli@sbmflab.org wrote: > >Hello, > >Need some help on determining cytoplasmic Kappa/Lambda ratio for plasma >cells. Can not decide which antibody combination is the best. > >Option #1 > Tube 1: Isotope K/L + CD45 > Tube 2: CD38/CD56/CD/45 > Tube 3: Kappa/Lambda/CD45 > > Gate on CD38 bright and CD56 positive population (plasma cells) and >determine K/L ratio > >Option #2 > Tube 1: Isotope K/L + CD45 > Tube 2: Kappa/CD38/CD45 > Tube 3: Lambda/CD38/CD45 > > Gate on CD38 bright (>10E3 log) and look at K & L fluorescence >intensity difference. How to determine the K/L ratio? > >May be neither of them are the best combination. Any helpful suggestion >will be appreciated. Looking forward to hearing from people soon. > > >Best Regards >Qing Li, Ph.D. >NTTD Department >South Bend Medical Foundation >South Bend, IN > > > > > >---------- > -- __________________________________________________________________ Your favorite stores, helpful shopping tools and great gift ideas. Experience the convenience of buying online with Shop@Netscape! http://shopnow.netscape.com/ Get your own FREE, personal Netscape Mail account today at http://webmail.netscape.com/
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