Hello, We use lambda/kappa/CD138 and gate on CD138/SSC. In four colour CD38 may be added. Moreover we run CD38/CD56/CD19 to determine normal vs pathological PC phenotype. Kind regards Anna At 08:38 2001-11-16 -0500, you wrote: > >Hello, > >Need some help on determining cytoplasmic Kappa/Lambda ratio for plasma >cells. Can not decide which antibody combination is the best. > >Option #1 > Tube 1: Isotope K/L + CD45 > Tube 2: CD38/CD56/CD/45 > Tube 3: Kappa/Lambda/CD45 > > Gate on CD38 bright and CD56 positive population (plasma cells) and >determine K/L ratio > >Option #2 > Tube 1: Isotope K/L + CD45 > Tube 2: Kappa/CD38/CD45 > Tube 3: Lambda/CD38/CD45 > > Gate on CD38 bright (>10E3 log) and look at K & L fluorescence >intensity difference. How to determine the K/L ratio? > >May be neither of them are the best combination. Any helpful suggestion >will be appreciated. Looking forward to hearing from people soon. > > >Best Regards >Qing Li, Ph.D. >NTTD Department >South Bend Medical Foundation >South Bend, IN > > > > > >---------- > Anna Porwit Hematopathology Lab. Department of Pathology, Radiumhemmet Karolinska Hospital, 171 76 Stockholm, Sweden Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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