Roger If i get your problem correctly, why not test to see if the T cells are coated with anti-human [=drug] antibody and just not responding?... that would at least answer some of the questions you have. use something like a goat anti-human that you know stains the drug. Make sure to add a CD19 to detect and distinguish normally 'coated' B cells. F Preffer At 06:54 PM 11/6/01 -0500, Roger D. Klein, M.D. wrote: > >I would like to repeat flow cytometry on the blood of >a patient with HTLV-1 T-Cell Leukemia/Lymphoma (CD 4+, >CD 25+), to assess CD52 status. The patient is currently >being treated with Campath - 1H, a humanized monoclonal >antibody that is reactive with CD52 (his disease was >found to be CD52+ in the past). The patient no longer >seems to be responding to Campath therapy,and his >disease now appears to be CD 52- by flow. I am concerned >that the Campath - 1 H (most recent dose yesterday) could >be masking any CD52 present on the patient's malignant cells. >Does anybody know of literature addressing this issue, or >otherwise have suggestions. Thank you very much for any >assistance that you can provide. > >Roger Klein, M.D. > Frederic I. Preffer Department of Pathology Charlestown Navy Yard- 7140 149 13th Street Massachusetts General Hospital -East Charlestown, MA 02129 voice [617] 726-7481 fax [617] 724-3164
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