Assessing CD52 status during Campath - 1H therapy

From: Roger D. Klein, M.D. (roger.klein@yale.edu)
Date: Tue Nov 06 2001 - 18:54:07 EST


I would like to repeat flow cytometry on the blood of
a patient with HTLV-1 T-Cell Leukemia/Lymphoma (CD 4+,
CD 25+), to assess CD52 status.  The patient is currently
being treated with Campath - 1H, a humanized monoclonal
antibody that is reactive with CD52 (his disease was
found to be CD52+ in the past).  The patient no longer
seems to be responding to Campath therapy,and his
disease now appears to be CD 52- by flow.  I am concerned
that the Campath - 1 H (most recent dose yesterday) could
be masking any CD52 present on the patient's malignant cells.
Does anybody know of literature addressing this issue, or
otherwise have suggestions.  Thank you very much for any
assistance that you can provide.

Roger Klein, M.D.



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