YO-PRO-1 works VERY well in distinguishing apoptotic cells. It makes dead cells highly fluorescent, apoptotic cells moderately fluorescent and live cells are dimly fluorescent or nonfluorescent. http://www.probes.com/handbook/figures/1517.html It has green fluorescence only when bound to nucleic acids and uses only FL1, leaving other channels available for other colors. It can also be used for imaging using fluorescein filters. The unbound dye is essentially nonfluorescent. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7561136&dopt=Abstract Theolis Barbosa wrote: > Hallo everybody, > > A colleague is interested in evaluating apoptosis in vitro and asked me > about ways to do it by FACS. He wouldn't like to use PI nor TUNEL, as he > had bad experiences with these methods. He asks whether DAPI/Hoechst > fluorescence could be used for detection of apoptotic nuclei by flow. In > this case, what compatible laser could be adapted to a FACSort? > If not, would there be any alternative fluorescent probes for detection of > apoptotic nuclei? > > ................................................................................................................................................ > > Theolis Barbosa, MSci > Laboratorio de Microbiologia e Imuno-regulação > Centro de Pesquisas Gonçalo Moniz - FIOCRUZ > R. Waldemar Falcão, 121 Brotas CEP 40295-001 > Salvador - Bahia - BRASIL
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