Hallo everybody, A colleague is interested in evaluating apoptosis in vitro and asked me about ways to do it by FACS. He wouldn't like to use PI nor TUNEL, as he had bad experiences with these methods. He asks whether DAPI/Hoechst fluorescence could be used for detection of apoptotic nuclei by flow. In this case, what compatible laser could be adapted to a FACSort? If not, would there be any alternative fluorescent probes for detection of apoptotic nuclei? ................................................................................................................................................ Theolis Barbosa, MSci Laboratorio de Microbiologia e Imuno-regulação Centro de Pesquisas Gonçalo Moniz - FIOCRUZ R. Waldemar Falcão, 121 Brotas CEP 40295-001 Salvador - Bahia - BRASIL
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