I hope that someone can help me with this problem as I am somewhat at a loss as to what to tell my client. He wants to do flow on embryonic mouse heart cells. He is staining for myosin heavy chain and for apoptosis with tunel. The problem is that I am unable to identify distinct populations(using forward and side scatter) within the cell suspension he is providing to me, and I am concerned that I am missing the important cells. I have tried looking at the ungated data for the myosin staining and have come no closer to finding populations of cells. I know that it is a mixed population of cells but expected to be able find populations with the mix. Another problems is that of course the hearts are really tiny and he is pooling many hearts together. I really appreciate any help anyone can give me with this situation. thanks in advance. Janet Dow Janet Dow Research Technician and Manager Flow Cytometry Facility North Carolina State College of Veterinary Medicine Room C-314 Raleigh, NC 27606 (919)513-6364
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