Hi,
thanks to all who provided input into this one. It seems that one person
has seen a DI of 0.5 hypodiploid and most have seen 0.8 or 0.9 D.I. which
is our past experience.
Image analysis showed a tetraploid population with no hypodiploid
population. Flow DNA from paraffin material showed a tetraploid population.
As we also troubleshooted everything, it appears that Chick RBCs were the
contaminant in the original frozen breast tissue that was processed. It
produced the D.I. of 0.41 with a doublet at 0.82 which made the histogram
appear to have a plausible hypodiploid population, except for the fact that
I had never heard of a hypodiploid with D.I. that low. It is good to
question and have back up methods to support a finding. It is also good to
have input from the community of flow cytometrists to add their knowledge
to your own special situations.
bessie
>
>Hi,
>Has anyone ever seen a breast cancer DNA Index of 0.41. We have a
>multiploid patient that shows DI of 0.41 (about 20% of the population ,
>0.82 (about 3% or so) 1.0 (about 20%), and 1.91(about 50%). I have never
>seen a hypodiploid that low. I am not sure what the 0.41 and 0.82 mean...
>looks like it could be a cycling population. CVs for the peaks are 2% or
>less. We confirmed the diploid population by adding back lymphocytes.
>Testing was done on frozen tissue with Krishan's PI reagent.
>
>I will follow up with image analysis and see how that goes, but I would
>like to know if anyone has seen a hypodiploid like this.
>
>thanks for the input
>bessie
>--
>Bessie Kam Email: bkam@dls.queens.org
>The Queen's Medical Center, Pathology FAX: 808-547-4045
> Phone: 808-547-4271
>
>
--
Bessie Kam Email: bkam@dls.queens.org
The Queen's Medical Center, Pathology FAX: 808-547-4045
Phone: 808-547-4271
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