I realize that the FACS Calibur multiwell autosampler is fairly new, but is anyone actively using one? Could you please share your successes and failures with it? Does it do what you need? What would you change about it? Would you recommend it to others? Is there anyone with experience at sorting cells using the FACS Calibur? I realize it has limitations, and I'm not expecting FACS Vantage-quality sorting. I have a cell line that is expressing a recombinant protein and I want to sort the highest protein expressors (e.g., top 1% or 10%) in that population. What is the likelihood that I could get 1000 - 2000 targeted cells at >98% purity after sorting for an hour or two? Carol Mazurek Millennium Pharmaceuticals, Inc. mazurek@mpi.com
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