I want to place one querry in front of the
forum.I hope that the forum will help me out.
I want to know that if I'm getting cell cycle arrest at one dose of any chemotoxic
agent & I want to see that at any other dose I'm getting increased apoptosis,Can
I couple the two techniques of BrdU labelling & Propidium iodide staining to see
this effect.Or more intricately,If I want to demarcate the apoptotic fraction
precursor population(as being S phase arrested or G2-M phase arrested) ,how
can I do this.
Or how can I know that which of population is going towards apoptosis(G0-G1
arrested or G2-M arrested population).
If somebody can help telling this,please reply me as soon as possible.
Thanx in advance.
Preeti
PhD student
India