Hi Preeti,
The APO-BRDU kit available from Pharmingen should work for you. You should be able
to get a horse shoe shaped profile on a dot plot of BrDU on the y-axis v/s PI on the
x-axis. The apoptotic cells will be sub G0/G1 and negative for BrDU. We have looked
at the effect of tyrosine kinase inhibitors on human myeloid cell lines and we were
able to see a G0/G1 arrest, followed by most of the cells undergoing apoptosis upon
longer incubation with the drug using this assay.
Vivek Tanavde
Johns Hopkins Comprehensive Cancer Center
Bunting-Blaustein Cancer Research Bldg, Room 268
1650 Orleans Street
Baltimore, MD 21231
I want to place one querry in front of the
forum.I hope that the forum will help me out.
I want to know that if I'm getting cell cycle arrest at one dose of any chemotoxic
agent & I want to see that at any other dose I'm getting increased apoptosis,Can
I couple the two techniques of BrdU labelling & Propidium iodide staining to see
this effect.Or more intricately,If I want to demarcate the apoptotic fraction
precursor population(as being S phase arrested or G2-M phase arrested) ,how
can I do this.
Or how can I know that which of population is going towards apoptosis(G0-G1
arrested or G2-M arrested population).
If somebody can help telling this,please reply me as soon as possible.
Thanx in advance.
Preeti
PhD student
India