Hi All, I don't think that FCS standard is a farce at all. The farce is that manufacturers don't adhere to it. Even if Bob's wildest dreams came true, and ISAC started recommending DICOM, and then the manufacturers started using only the bits of the standard that appealed to them, then the DICOM "standard" would not be worth much. The FCS standard specifies fields for all of the information that is put in the BDWORD fields and the fact that BD does not use them is a disservice to the flow community at large, considering that most software will not know how to deal with them. and in theory can change them at will. Maybe there are some limitations to the FCS Standard in terms of validation and tracking changes to the data but I think it does its job pretty well. Although maybe my bias is somewhat tainted by not wanting to write a new FCS data parser for FCS Express :-) -Dave At 09:38 AM 10/31/01 +1000, you wrote: Dear All, (As an aside, it nicely demonstrated what a mess (is farce too harsh a term?) the FCS "standard" has become, and backs up Bob Leif's recent comments regarding the inadequacies of the Flow Cytometry Standard.) At 09:38 AM 10/31/01 +1000, Geoffrey Osborne wrote: >Dear All, > Following the number of replies I had regarding the BD$WORD > values in the >text section of CellQuest ProŠ 4.0.1 generated FCS files, I thought our >findings might be of interest. (As an aside, it nicely demonstrated what a >mess (is farce too harsh a term?) the FCS "standard" has become, and backs >up Bob Leif's recent comments regarding the inadequacies of the Flow >Cytometry Standard.) > On our LSR system the following holds true. >BD$WORD3 P2 voltage >BD$WORD5 P3 voltage >BD$WORD7 P4 voltage >BD$WORD9 P5 voltage >BD$WORD11 P6 voltage >BD$WORD55 P7 voltage >BD$WORD24 P8 voltage > >For compensation, the formula is: (field value/250) * 100, >thus for example in BD$WORD12 (FL1 - %FL2) a change from (10% -> 20%) >compensation results in a field value change of 25 -> 50 >(10% -> 30%) : 25 >-> 75 >(10% -> 40%) : 25 -> 100 >(10% -> 50%) : 25 -> 125 >(10% -> 59.6%) : 25 >-> 149 > >BB$WORD12 (FL1 - FL2) >BB$WORD13 (FL2 - FL1) > >BD$WORD16 (FL1 - >FL3) >BD$WORD17 (FL3 - FL1) > >BD$WORD18 (FL1 - FL6) >BD$WORD19 (FL6 - >FL1) > >BD$WORD20 (FL2 - FL6) >BD$WORD21 (FL6 - FL2) > >BD$WORD22 (FL3 - >FL6) >BD$WORD23 (FL6 - FL3) > >BD$WORD28 (FL2 - FL3) >BD$WORD29 (FL3 - >FL2) > >BD$WORD44 (FL4 - FL5) >BD$WORD45 (FL5 - FL4) > >Hope this helps, >Geoff >Geoffrey Osborne > >Specialist, Flow Cytometry, >John Curtin School of Medical Research, >The Australian National University, >Canberra, 0200, ACT. AUSTRALIA >email: geoff.osborne@anu.edu.au >http://jcsmr.anu.edu.au/facslab/facshome.html > >(61 2) 6125 3694.
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