Dear All, Following the number of replies I had regarding the BD$WORD values in the text section of CellQuest ProŠ 4.0.1 generated FCS files, I thought our findings might be of interest. (As an aside, it nicely demonstrated what a mess (is farce too harsh a term?) the FCS "standard" has become, and backs up Bob Leif's recent comments regarding the inadequacies of the Flow Cytometry Standard.) On our LSR system the following holds true. BD$WORD3 P2 voltage BD$WORD5 P3 voltage BD$WORD7 P4 voltage BD$WORD9 P5 voltage BD$WORD11 P6 voltage BD$WORD55 P7 voltage BD$WORD24 P8 voltage For compensation, the formula is: (field value/250) * 100, thus for example in BD$WORD12 (FL1 - %FL2) a change from (10% -> 20%) compensation results in a field value change of 25 -> 50 (10% -> 30%) : 25 -> 75 (10% -> 40%) : 25 -> 100 (10% -> 50%) : 25 -> 125 (10% -> 59.6%) : 25 -> 149 BB$WORD12 (FL1 - FL2) BB$WORD13 (FL2 - FL1) BD$WORD16 (FL1 - FL3) BD$WORD17 (FL3 - FL1) BD$WORD18 (FL1 - FL6) BD$WORD19 (FL6 - FL1) BD$WORD20 (FL2 - FL6) BD$WORD21 (FL6 - FL2) BD$WORD22 (FL3 - FL6) BD$WORD23 (FL6 - FL3) BD$WORD28 (FL2 - FL3) BD$WORD29 (FL3 - FL2) BD$WORD44 (FL4 - FL5) BD$WORD45 (FL5 - FL4) Hope this helps, Geoff Geoffrey Osborne Specialist, Flow Cytometry, John Curtin School of Medical Research, The Australian National University, Canberra, 0200, ACT. AUSTRALIA email: geoff.osborne@anu.edu.au http://jcsmr.anu.edu.au/facslab/facshome.html (61 2) 6125 3694.
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