Hi folks. Can anyone point me to a discussion of ways to estimate the purity of a sorted population of cells. This is clearly easy when you are sorting very bright cells from negatives but more problematic when sorting cells from a "shoulder". I remember seeing a nice discussion on this subject but cannot remember where. I've been sorting some very weakly positive cells from a population, these cells post sort overlap the negative cells quite a bit. My customer seems unhappy about that. Thanks Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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