We routinely purify rare (0.1% - 5%) populations from bone marrow by staining the cells with the relevant PE or PE-tandem dye conjugated ab's then select with anti-PE microbeads. You don't need to stain with a second ab to check for purity (handy if there aren't multiple abs to several epitopes available) and we usually get >90% purity. Bit more costly though. Nick. W. Nicholas Haining, B.M., B.Ch. Pediatric Oncology/Hematology Dana Farber Cancer Institute -----Original Message----- From: Kong, Denice [SMTP:KongD@surgery.ucsf.edu] Sent: Thursday, October 18, 2001 5:01 PM To: Cytometry Mailing List Subject: Miltenyi magnetic beads and CD34+ Dear flow users: I am currently evaluating using Miltenyi microbeads to isolate CD34 from peripheral blood and checking the purity using flow. Has anyone done this type of study before. If so, where would the CD34+ cells be on the SSC and FSC plot with or without the microbeads? What purity was obtained? Denice Kong UCSF-ITL
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