Andy, What lasers do you have ? and how many detectors off each ? or is this for a new instrument ? Rachel ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Andy Johnson > Sent: Monday, October 22, 2001 1:33 PM > To: Cytometry Mailing List > Subject: 6 colour sorting > > > Dera Flowers > > How many people are using 6 colours on a Vantage ? 3 colours off the 488, > APC plus APC-Cy7 (very weak) with the 633 and Hoechst is fine off the UV. > Unfortunately I need colours such as cascade blue/yellow and something > brighter than the APC-Cy7. > > Can anybody who uses more than the standard colours give me a suggestion > as > to how to either increase sensitivity or which colours work best. I also > have a feeling that it maybe an issue between the Vantage and the MoFlo > (being more sensitive). I know all the available fluorochromes out there, > so what I need to know is which one people have used and got to work. > > Would a 120mW from a I-70 laser greatly increase the UV signals, or should > the 60mW from an enterprise be sufficient ? > > Hoping that somebody can help ? > > Andy > > >
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