To anybody in the field of cytometry and T cell immunology if you are interested in recent developments in flow cytometry, especially analysis and sorting of antigen-specific T cells, multiparameter cytokine detection in live and fixed cells or sensitive analysis of low receptor numbers we would like to announce the: I. Florence-Berlin Cytometry School: "Antigen-specific T cell cytometry" March 20-23, 2002, Florence, Italy Restricted to 25 participants Infos and registration: www.dmi.unifi.it/cytometry-school/general.htm Topics ANALYSIS AND ISOLATION OF CYTOKINE SECRETING CELLS (Mario Assenmacher, Miltenyi Biotec, Bergisch Gladbach, Germany) MHC-CLASS I TETRAMERS (Dirk H. Busch, Institute for Microbiology, Technical University Munich, Germany) MULTIPARAMETER CYTOKINE ANALYSIS OF ANTIGEN-SPECIFIC T CELLS (Holden Maecker, BD Biosciences, S. Jose, USA) CYTOMETRY OF PROLIFERATING CELLS (Lorenzo Cosmi, DMI, University of Florence, Italy) MULTIPARAMETER CALCIUM INFLUX ANALYSIS USING BD-LSR (Francesco Annunziato, DMI, University of Florence, Italy) HIGH-SENSITIVITY IMMUNOFLUORESCENCE (Alexander Scheffold, DRFZ Berlin, Germany) ____________________________________________ Alexander Scheffold, PhD Deutsches Rheumaforschungszentrum Berlin Immunocytometry Group Schumannstr. 21/22 (visitors: Virchow-Weg 12) 10117 Berlin Tel.: +49-30-28460-0/-700 FAX: +49-30-28460-603 e-mail:scheffold@drfz.de
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