Dera Flowers How many people are using 6 colours on a Vantage ? 3 colours off the 488, APC plus APC-Cy7 (very weak) with the 633 and Hoechst is fine off the UV. Unfortunately I need colours such as cascade blue/yellow and something brighter than the APC-Cy7. Can anybody who uses more than the standard colours give me a suggestion as to how to either increase sensitivity or which colours work best. I also have a feeling that it maybe an issue between the Vantage and the MoFlo (being more sensitive). I know all the available fluorochromes out there, so what I need to know is which one people have used and got to work. Would a 120mW from a I-70 laser greatly increase the UV signals, or should the 60mW from an enterprise be sufficient ? Hoping that somebody can help ? Andy
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