At 7:57 AM -0500 19/10/2001, Michael Herron wrote: >All, > >I am using biotinylated rat anti-mouse CD4 and CD8 from Pharmingen to do >immunohistochemistry on frozen sections of Balb/c scid NCr mice from >NCI. This was SUPPOSED to be a negative control, but these mice are >staining positively in the spleen for CD4 and CD8. > >I have observed this in mice 7-12 weeks old. Other tissues like kidney >are negative. These mice are also ab-TCR negative. > >When assayed by FACS these mice do not show the same large populations >of CD4 or CD8 positive cells as with IHC. > >NCI doesn't seem to have any record of this occurring, and these mice >should be too young to be "leaky" as has been reported elsewhere. > >Has anyone seen this before? Does anyone have any ideas or possible >explanations? > >Thanks, >Mike Subsets of splenic dendritic cells can express CD4 or CD8. Adrian Vremec, D., J. Pooley, H. Hochrein, L. Wu and K. Shortman. (2000) CD4 and CD8 expression by dendritic cell subtypes in mouse thymus and spleen. Journal of Immunology 164, 2978-86. Vremec, D. and K. Shortman. (1997) Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes. Journal of Immunology 159, 565-73. Vremec, D., M. Zorbas, R. Scollay, D. J. Saunders, L. W. C.F. Ardavin and K. Shortman. (1992) The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. The Journal of Experimental Medicine 176, 47-58.
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