Hi Richard, It is my impression that whenever a real DNA-tetraploid (G1) population is present, these cells will further proceed through their cell cycle, with DNA-synthesis (towards 8n DNA) and G2/M (8n DNA) being apparent. So if you see any events above 4n, my guess is that the 4n cells represent a happy tetraploid G1 population, whereas if there's nothing above 4n, then your tetraploid population is most probably a blocked G2/M population (especially if its % is increased). I hope this helps? Best regards, Dirk Prof. Dirk Van Bockstaele Laboratory of Hematology Head of Flow Cytometry / Molecular Diagnostics Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium phone 32 3 821 3900 fax 32 3 825 1148 > ---------- > Van: Salinas, Richard[SMTP:rsalinas@saci.org] > Verzonden: donderdag 18 oktober 2001 19:26 > Aan: Cytometry Mailing List > Onderwerp: Tetraploid populations > > > Greetings all, > > My question pertains to DNA cell cycle analysis in frozen tumors. > By definition, tetraploid peaks have DI's from 1.9 - 2.1 with the > proportions of cells greater than that of the normal diploid G2/M > population. > In samples, when the diploid S phase is usually low, I would expect a > somewhat low G2/M as well, but when I see a G2/M of 40% I immediately > think > tetraploid. > Is there a clear cut way to distinguish and validate normal G2/M cells vs. > Tetraploid populations? > I ask for your enlightenment. > > Richard A. Salinas > > Flow Cytometry Laboratory > Dept. of Radiation Oncology > Texas Research Park > San Antonio, TX 78245 > (210) 677-3882 > > >
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