All,
I am using biotinylated rat anti-mouse CD4 and CD8 from Pharmingen to do
immunohistochemistry on frozen sections of Balb/c scid NCr mice from
NCI. This was SUPPOSED to be a negative control, but these mice are
staining positively in the spleen for CD4 and CD8.
I have observed this in mice 7-12 weeks old. Other tissues like kidney
are negative. These mice are also ab-TCR negative.
When assayed by FACS these mice do not show the same large populations
of CD4 or CD8 positive cells as with IHC.
NCI doesn't seem to have any record of this occurring, and these mice
should be too young to be "leaky" as has been reported elsewhere.
Has anyone seen this before? Does anyone have any ideas or possible explanations?
Thanks,
Mike
--
________________________________________________________
/ Michael J. Herron, U of MN, Dept. of Pediatrics/BMT /
/ herro001@umn.edu /
/ 612-626-4321 Mpls MN 55455 /
/_______________________________________________________/
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