Re: Mouse CD4+ splenocytes

From: Oberdan Leo (oleo@dbm.ulb.ac.be)
Date: Wed Oct 10 2001 - 04:10:40 EST


>

Dear Jan,

CD8+ cells do proliferate better than CD4+ unders your experimental
conditions. If you need a cell population comprsing more than 50 %
activated CD4+, it is better to get rid of CD8+ (by Ab + complement or
negative selection) before the culture.

Good luck,

Oberdan LEO




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