Hi list, I would like to learn how to efficiently stimulate CD4 positive lymphocytes from mouse spleen. I tried to do that using ConA and/or PHA + IL2, but that will only give me some thirty percent or so CD4 positives after one week of culture. When stimulating human peripheral blood lymphocytes using PHA + IL2, we get at least 50 percent of CD4 positives after one week. Thank you,
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