Dear flow users, I would like your opinion to a situation concerning fixation of cells for DNA analysis. We are working with a number of human cancer cell lines in the study of cell cycle and apoptosis. We have found that a 1 hour fixation with cold EtOH is not enough to obtain an adequate DNA profile. We have, to be sure that the protocol is consistantly followed, fixed the cells overnight. I think That 1 hour is not enough because we see massive variations in the instrument settings of the Calibur. The cells are stained with PI. I would really appreciate some feedback to this opinion. Thank you in advance, Marylou Zuzarte FACS & Confocal Facility Prof. Rolf Muller, Inst. f. Molekularbiologie & Tumorforschung Emil-Mannkopf Str 2 35037 Marburg, Germany Tel: 49-(0)6421-2865925 Zuzarte@IMT.uni-marburg.de
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