We have recently been receiving samples from a town far enough away from us that the samples spend up to 30 or more hours in transit. We have sent them RPMI + 10% FCS (and an antibiotic) to place the samples in for transport. By the time we receive the samples the pleasant fruit punch colored RPMI has changed to a disgusting lemon yellow (very acidic) and the viability of the cells has dropped to less than 20%. What's wrong with this picture? The samples have been fairly large; do we need to just have them use more containers and split the sample into smaller portions? Or is there some other issue we are missing? Would someone who has experience in this transport issue please let me in on the secret to long distance cell viability! Thanks so very much. Kathy Altig Southwest Washington Medical Center Vancouver, WA
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