Hi Flowers! As a novice in the field of apoptosis I wish to get a few opinions from some of you experts. We wish to study apoptosis in whole blood after stimulation with e.coli. We have a standard FACSCalibur with one laser (488 nm) and I indeed like simple assays with minimal handling of the samples. Tips of any commercial kits or antibodies would also be welcomed. Which early marker of apoptosis would be suitable? Detection of caspase activity or trans membrane potential or something else? Does anyone know the timeaspects from here to the ability of binding annexin, as I understand would be an easy marker to study together with PI? Is annexinbinding actually a late marker? And if we wish to study a late marker what would then be suitable, when taking into consideration the ability to distinguish between necrosis and late apoptosis? Regards Dorte Christiansen Research lab Nordland Central Hospital Bodø, Norway
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