Dr. Christiansen inquired about choice of a simple method to assess early and late apoptosis. We have now good experience with the FLICA assay that detects activation of caspases (See Smolewski et al., Cytometry, 44: 73-82, 2001). The major advantage of FLICA is that these reagents also can be used to arrest the process of apoptosis and therefore allow one to assess cumulative apoptotic index (Smolewski et al., Stathmo-apoptosis: arresting apoptosis by fluorochrome-labeled inhibitor of caspases. Int. J. Oncology, 19:657-663,2001). Thus, both, early and late apoptotic cells can be detected at the same time. Zbigniew Darzynkiewicz Brander Cancer Research Institute New York Medical College 19 Bradhurst Ave. Hawthorne, NY 10532 tel: 914-347-2801 fax: 914-347-2804 http://www.geocities.com/z_darzynkiewicz
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