Hi Anne, I am currently working with our BD rep on this same issue. We have successfully analyzed the results on our PC using their CBA software, but the samples were acquired on our FACS Calibur. I can't understand why the software doesn't analyze the data acquired on the Coulter XL. I will share my solution when resolved. Regards, Paul Hallberg CORE FLow Cytometry Facility Kimmel Cancer Center Thomas Jefferson University -----Original Message----- From: Anne C Avery [mailto:anne.avery@colostate.edu] Sent: Wednesday, August 15, 2001 4:27 PM To: cyto-inbox Subject: Cytokine bead assay from Pharmingen Hello, I was wondering if anyone has used the new cytokine bead assay from BD/Pharmingen (mouse Th1/Th2 kit) to quantify cytokines. In particular, all of the support materials address data analysis with BD instruments and software (not surprisingly). We have a Coulter XL, and do not have CellQuest. I would be intereested in hearing from anyone who as anlalyzed the data generated with these reagents as to whether it can be done using software other than CellQuest and the package available from Pharmingen (which apparently only works with CellQuest). Anyone from BD is welcome to weigh in... Tbanks very much. Anne ==================================== Anne Avery, VMD, PHD Department of Pathology Colorado State Univ Ft. Collins, CO 80523-1671, USA voice:(970)491-1170 fax:(970)491-0603 anne.avery@colostate.edu ====================================
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