Cytokine bead assay from Pharmingen

From: Anne C Avery (anne.avery@colostate.edu)
Date: Wed Aug 15 2001 - 15:26:58 EST


Hello,

I was wondering if anyone has used the new cytokine bead assay from
BD/Pharmingen (mouse Th1/Th2 kit) to quantify cytokines.  In
particular, all of the support materials address data analysis with BD
instruments and software (not surprisingly).  We have a Coulter XL, and
do not have CellQuest.  I would be intereested in hearing from anyone
who as anlalyzed the data generated with these reagents as to whether
it can be done using software other than CellQuest and the package
available from Pharmingen (which apparently only works with CellQuest).
Anyone from BD is welcome to weigh in...

Tbanks very much.

Anne

====================================
Anne Avery, VMD, PHD
Department of Pathology
Colorado State Univ
Ft. Collins, CO 80523-1671, USA
  voice:(970)491-1170
  fax:(970)491-0603

anne.avery@colostate.edu
====================================



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