MHC-Tetramer Assay

From: PAUL HALLBERG (Paul.Hallberg@mail.tju.edu)
Date: Tue Aug 07 2001 - 10:56:32 EST

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Hi FLOWers,

Can anyone explain why APC-SA gives a better S/N ratio than PE-SA for some
MHC Tetramer Assays? In addition, does anyone know an alternative
fluorochrome conjugated to strepavidin using 488nm laser excitation?

Thanks in advance,

Paul L. Hallberg
CORE Flow Cytometry Facility
Kimmel Cancer Center
Thomas Jefferson University

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