Dear Paul, Perhaps I can not explain the phenomenon you see with the APC conjugates but at least I can confirm the same findings with some of our tetramers. We have seen that APC sometimes functions better then PE with certain T cell populations. We think that it might be due to the difference in size of the final constructs, on the other hand it has been suggested that APC form larger multimers then 4 (although without evidence). I am curious to the experience of other MHC-tetramer users, more information on our tetramer facility can be found on our web site www.clb.nl <http://www.clb.nl> and go to "reagents". Best regards, John John Voorn CLB Reagents Productmanager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: PAUL HALLBERG [SMTP:Paul.Hallberg@mail.tju.edu] Sent: Tuesday, August 07, 2001 5:57 PM To: Cytometry Mailing List Subject: MHC-Tetramer Assay Hi FLOWers, Can anyone explain why APC-SA gives a better S/N ratio than PE-SA for some MHC Tetramer Assays? In addition, does anyone know an alternative fluorochrome conjugated to strepavidin using 488nm laser excitation? Thanks in advance, Paul L. Hallberg CORE Flow Cytometry Facility Kimmel Cancer Center Thomas Jefferson University
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