Ellen: Many people that I know perform annexin V on adherent cells. This is not a good idea as you have brought up. In general it is probably consisdered bad science. However, ICT sells a cellular release reagent to circumvent the problem of popping holes in the membrane of adherent cells. I have not treid it. If one really wants to run Annexin on adherent cells than they had better run strict controls to know what the level of false positives are being produced. This is cell type dependent as you would image. Certainly if it is possible, one should try to avoid annexin on adherent cells. I am curious to hear other peoples comments on this topic. Scott Tighe Vermont Cancer Center Ellen.Freed@astrazeneca.com wrote: > > Since we're on this subject. I've been told it's an early marker. . . > I have a separate question. Is anyone doing Annexin V staining on adherent > cells. I understand that once you trypsinize the cells, this is no longer > an accurate measure. Any comments on this or ways to get around it? > > Thanks > > Ellen Freed > > Ellen Freed > Scientist > AstraZeneca R&D Boston
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