Hi Nancy, Rest assured, it'll fluoresce better than you'd imagine it would from it's reputation, in fact it'll work better than you'd want it to if you were hoping to separate its fluorescence from any of the other GFP spin-offs (which is the only time you'll need to resort to differential excitation). Ray > From: "Nancy Harvey" <nharvey@microbio.umsmed.edu> > Date: Mon, 23 Jul 2001 11:00:37 -0500 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Can EYFP be run on FACScan? > > > Hello, > I have an investigator who wants to check samples that have enhanced YFP. The > ideal > excitation is 514. Has anyone been able to detect EYFP with the 488 > excitation of > a FACScan? > > I could tune the primary laser on the Vantage to 514, but have only 488/10 > filters > for FSC and SSC. > > Any thoughts and suggestions are appreciated. > > Nan Harvey > University of MS Medical Center > Jackson, MS 39216 > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:26 EST