Nancy, We've done EYFP on our Elite sorter using an air-cooled argon 488 nm line, about 15 mW. So, I would suspect this would also work on your Scan. MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> "Nancy Harvey" <nharvey@microbio.umsmed.edu> 07/23/01 12:00PM >>> Hello, I have an investigator who wants to check samples that have enhanced YFP. The ideal excitation is 514. Has anyone been able to detect EYFP with the 488 excitation of a FACScan? I could tune the primary laser on the Vantage to 514, but have only 488/10 filters for FSC and SSC. Any thoughts and suggestions are appreciated. Nan Harvey University of MS Medical Center Jackson, MS 39216
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