YFP is readily detectable using 488 ex. and FL1 on the FACScan ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX) > ---------- > From: Nancy Harvey > Sent: Monday, July 23, 2001 12:00 PM > To: Cytometry Mailing List > Subject: Can EYFP be run on FACScan? > > > Hello, > I have an investigator who wants to check samples that have enhanced YFP. > The ideal > excitation is 514. Has anyone been able to detect EYFP with the 488 > excitation of > a FACScan? > > I could tune the primary laser on the Vantage to 514, but have only 488/10 > filters > for FSC and SSC. > > Any thoughts and suggestions are appreciated. > > Nan Harvey > University of MS Medical Center > Jackson, MS 39216 > >
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