Hi, I am interested in a response from anyone who has flow experience on bacteria or microsporidia that measure one micron in size. We have successfully performed flow (FACScan) on microsporidia that measure two microns, but this was close to the minimum size limit on the FSC profiles when using LOG mode. The purified populations become more dispersed when LINEAR mode is used on the scatter profiles of which we prefer not to use. Is it better to use LINEAR or LOG mode for scatter on one micron organisms using a FACscan? Thanks in advance. Delynn Moss Centers for Disease Control Division of Parasitic Diseases
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