Hello Flowers: I wonder if any one has some experience with the kappa /lambda preparation on permeabilized cells ( Intraprep) for determinig cytoplasmic expression of the light chains and hence clonality ( restriction) in B cells. It seems that occasionally, a preparation artifact occurs where only one light chain is expressed in otherwise polyclonal populations of B cells. Commonly we use the Intraprep when surface labeling is very dim or negative. The common combination is CD45/CD19/kappa/lambda. The CD19 is ususally the fourth color-PC5 I would appreciate some comments on this problem Thanks.
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