Interesting story. What receptor were you transfecting in. In some cases, the antibody might activate a death response. This happens with HER2/herceptin. Tim Kute JASON MCCORMICK wrote: > Hello all. > > Has anyone out there had the experience of a transfected-in surface receptor inducing > post-sort death? > Got a cell line which is just peachy for shoving in other, quasi-related surface > receptors, but it doesn't seem to like this one. The pre-sort viability is okay (>80% > even at high culture density), but after staining, washing, sorting, the viability > drops to less than 1%. Have verified that sort recovery is good by counting (oh, > my aching retinas), but the darn things just die. > > Any ideas? > > Thanks in advance, > > Jason
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