Hello all. Has anyone out there had the experience of a transfected-in surface receptor inducing post-sort death? Got a cell line which is just peachy for shoving in other, quasi-related surface receptors, but it doesn't seem to like this one. The pre-sort viability is okay (>80% even at high culture density), but after staining, washing, sorting, the viability drops to less than 1%. Have verified that sort recovery is good by counting (oh, my aching retinas), but the darn things just die. Any ideas? Thanks in advance, Jason
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