Hi! I'm using a BD FACS Calibur with the (transparent) BD Falcon tubes for many years. However, last week i tested some (cheaper and more opaque) Sarstedt tubes in comparison to the Falcons and I got totally different results in murine T-cell expression of CD25, CD69 (positive vs. negative) and CD62L (high vs. low) in the same cells only correlated to the tubes. Does anyone have an explanation for this or found similar phenomens? So never change a winning team or shold one choose the tubes which will give the expected results? Any suggestions will be welcome. Thnks a lot R. Ruckert Research Center Borstel
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