My guess is that the population of cells that seems to be reduced adhere better to one tube versus the other. You could always try coating the cheaper tubes with BSA, but then are they still a bargain? David McFarland GlaxoSmithKline ---------------------- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 07/09/2001 09:30 --------------------------- rrueckert@fz-borstel.de on 28-Jun-2001 14:23 Please respond to rene@rueckert.as To: cyto-inbox cc: Subject: FACS tubes Hi! I'm using a BD FACS Calibur with the (transparent) BD Falcon tubes for many years. However, last week i tested some (cheaper and more opaque) Sarstedt tubes in comparison to the Falcons and I got totally different results in murine T-cell expression of CD25, CD69 (positive vs. negative) and CD62L (high vs. low) in the same cells only correlated to the tubes. Does anyone have an explanation for this or found similar phenomens? So never change a winning team or shold one choose the tubes which will give the expected results? Any suggestions will be welcome. Thnks a lot R. Ruckert Research Center Borstel (See attached file: rrueckert.vcf)
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:24 EST