SYTO red and HOECHST or DAPI

From: Elena Soriano (E.Soriano@iam.boku.ac.at)
Date: Wed Jun 06 2001 - 05:20:21 EST 

My intention is to use SYTO red for RNA specific staining like I use
Pyronine- Y. Does anyone knows if this possible?

When I stain the DNA with Hoechst or DAPI (selective for DNA), the
Pyronine-Y finds no place in these molecules, and therefore binds only to
the RNA, wich is then the only nucleic acid that has free binding points.

-> Could I block the DNA with Hoechst or DAPI and then stain with SYTO red
to detect the RNA?

Would SYTO red still find free binding points when the DNA is allready
stained with one of these DNA selective fluorochromes?

Thanks for your attention!

Elena.




-----------------------------------------
Elena Soriano
c/o Institut für Angewandte Mikrobiologie
    Univ.f.Bodenkultur Wien

Tel: +43 1 36006 6241
Fax: +43 1 36 97 615
http://www.boku.ac.at/iam

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