Elena, I think a paper worth looking at, which is relevant to your question, is K.Toba et al./Journal of Immunological methods 182 (1995) 193-207. They compared five "DNA/RNA" dyes (PI,7AAD,AO,PY,TO) and for stated voltages and compensation settings on a particular instrument provide a table for comparison of emission spectra and nucleic acid binding characteristics. Hope this helps Geoff At 12:20 PM 6/6/01 +0200, you wrote: > >My intention is to use SYTO red for RNA specific staining like I use >Pyronine- Y. Does anyone knows if this possible? > >When I stain the DNA with Hoechst or DAPI (selective for DNA), the >Pyronine-Y finds no place in these molecules, and therefore binds only to >the RNA, wich is then the only nucleic acid that has free binding points. > >-> Could I block the DNA with Hoechst or DAPI and then stain with SYTO red >to detect the RNA? > >Would SYTO red still find free binding points when the DNA is allready >stained with one of these DNA selective fluorochromes? > >Thanks for your attention! > >Elena. > > > > >----------------------------------------- >Elena Soriano >c/o Institut für Angewandte Mikrobiologie > Univ.f.Bodenkultur Wien > >Tel: +43 1 36006 6241 >Fax: +43 1 36 97 615 >http://www.boku.ac.at/iam Geoffrey Osborne Specialist, Flow Cytometry, John Curtin School of Medical Research, The Australian National University, Canberra, 0200, ACT. AUSTRALIA email: geoff.osborne@anu.edu.au http://jcsmr.anu.edu.au/facslab/facshome.html (61 2) 6125 3694.
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