Hello, I am trying to help a colleague look at viability in sperm on my FACScan. Setting the FSC and the SSC detectors has been a bit difficult because there are apparently very heterogenous populations in the different parts of the epididymis and I do not have a modification to align the sperm like on the MoFlow. Has anyone out there done anything with sperm on a FACScan? If so, can you recommend settings to use? Also, has anyone experience with SYBR14 dye and sperm from Molecular Probes? Thanks very much in advance. Beverly Barton Department of Surgery UMDNJ-New Jersey Medical School Newark, NJ USA
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