Here are some reported volumes in cubic microns.
Am J Pediatr Hematol Oncol 1983 Summer;5(2):181-8
Comparative studies of functional characteristics
of mononuclear cell subsets and
granulocytes.
Boxer LA, Yasaka T, Butterick CJ, Tzeng DY, Baehner
RL.
Two human peripheral blood monocyte subsets and
lymphocytes were isolated by counterflow centrifugal elutriation
(CCE). The cell volumes of 303 mu3 and 380 mu3 were
measured for the smaller and larger monocyte populations,
respectively. Superoxide release by large monocytes
exposed to opsonized zymosan was five times more active
than that of the small monocytes. The production of
colony stimulating activity was two-fold greater and the
myeloperoxidase activity was 1.4-fold greater by
the larger monocytes. Enriched fractions of cytotoxic cells
responsible for natural killer (NK) activity
against neuroblastoma cells were also obtained by counterflow centrifugal
elutriation. Natural killer cells were obtained in
larger lymphocyte fractions and had a mean cell volume of 180 mu3.
Compared with the NK activity against the
neuroblastoma cells, both the small and large monocytes displayed
greater antibody-dependent cellular cytotoxicity
(ADCC) activity against human erythrocytes. The larger peripheral
blood monocytes aggregated in response to FMLP
(N-formyl-methionyl-leucyl-phenyl alanine peptide) and PAF
(platelet activating factor). Unlike the
granulocyte, monocyte aggregation in response to FMLP was not
accompanied by degranulation nor was it potentiated
by cytochalasin B. In addition, monocyte aggregation could
be blocked by benoxaprofen, unlike the granulocyte.
Thus, CCE provides a means of isolating subsets of
monocytes and lymphocytes and obtaining large
numbers of peripheral blood monocytes for functional studies.
2: J Immunol 1981 Oct;127(4):1515-8
Functions of human monocyte and lymphocyte subsets
obtained by countercurrent
centrifugal elutriation: differing functional
capacities of human monocyte subsets.
Yasaka T, Mantich NM, Boxer LA, Baehner RL.
Two monocyte subsets from the peripheral blood of
healthy humans were isolated by countercurrent centrifugal
elutriation (CCE), and their functional
heterogeneity was assessed. Cell volume of the smaller monocyte population
was (332 +/- 32 mu 3), compared with the larger
monocytes (380 +/- 30 mu 3). Superoxide release by large
monocytes exposed to opsonized zymosan was 1.5-fold
more active than those of small monocytes. Furthermore,
the production of colony-stimulating factor (CSF)
and myeloperoxidase activity by the larger monocytes was 2-fold
and 1.4-fold greater than that of small monocytes,
respectively. Thus, CCE provides a useful means to isolate 2
subsets of monocytes with similar functional
properties but quantitatively different activities. This suggests that the
2 subsets may be closely related in their
developmental spectrum.
"Gaffin, Stephen L USARIEM" wrote:
> Can someone tell me the normal reference ranges of the volumes in
> femtoliters of
> human adult lymphocytes and monocytes? and/or reference?
>
> Thank you
> Steve
>
> *************************************************************
> *************************************************************
> ** Stephen L. Gaffin, Ph.D. **
> ** Research Physiologist **
> ** U.S. Army Research Institute for Environmental Medicine **
> ** Natick MA 01760-5007 **
> ** Tel. 508-233-4867 Fax 508-233-5298 **
> ** email stephen.gaffin@na.amedd.army.mil **
> *************************************************************
> *************************************************************
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