We have been studying the levels of apoptosis in peripheral blood mononuclear cells of patients with paracoccidioidomycosis. These cells are cultivated for 4-7 days with a specific fungal antigen, and we are assessing the apoptosis through Annexin-V and PI. Do someone know how to interpret those cells that are double stained (FITC+PI): cells that are in late apotpsosis process? Necrosis? In our cultures we detect very few cells stained with PI only, but significant numbers of cells stained with FITC only, double stained cells, or (the majority, live) non-stained cells.
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