We have also observed four kinds of sperm (bull) populations stained with Annexin V and PI, based on their fluorescence, i.e. green, red, double (green+red) and no fluorescence. We categorize these double stained as "Early Necrotic" sperm. ********* M. Anzar DVM, PhD Department of Animal and Poultry Science University of Guelph Guelph, ON, N1G 2W1 Canada ********* Camila Rodrigues Cacere wrote: > > We have been studying the levels of apoptosis in peripheral blood > mononuclear cells of patients with paracoccidioidomycosis. These cells > are cultivated for 4-7 days with a specific fungal antigen, and we are > assessing the apoptosis through Annexin-V and PI. > Do someone know how to interpret those cells that are double stained > (FITC+PI): cells that are in late apotpsosis process? Necrosis? In our > cultures we detect very few cells stained with PI only, but significant > numbers of cells stained with FITC only, double stained cells, or (the > majority, live) non-stained cells. -- ÐÏࡱá
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