Dare I wade into this? I just wanted to say that one can always sort at faster and faster rates. The purity may alway stays good (on any cytometer) until the cells start to coincide in the laser beam. What falls is the recovery. And assessing recovery is not easy --- partly because counting cells with accuracy is difficult and partly because the definition of "recovery" varies. We may get good recovery based on the number of cells that the instrument says it has sorted. But that might not be good recovery based on the number of cells that have gone through the analysis point. Some people use the words "yield" and "recovery" to distinguish these two aspects of sort assessment. One real problem is that very few labs have different sorters side by side --- so real comparisons are difficult..... Alice Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:19 EST