We have had excellent results using the Pharmingen activated caspase-3 reagent (#559341). It is quite sensitive and works well with adherent cell lines. Phil Marder ----Original Message Follows---- From: Anke Kretz <kretz-rommel@alxnsd.com> Reply-To: kretz-rommel@alxnsd.com To: cyto-inbox Subject: apoptosis assay on adherent cells Date: Fri, 18 May 2001 13:15:41 -0700 I am trying to determine apoptosis induction on adherent tumor cells. I used annexin/PI staining, however the background of annexin staining in untreated cells was up to 20 %. I am concerned that the cell dissociation buffer already induces apoptosis. I also tried IHC, but was loosing a lot of cells when I was trying to fix them. Does anybody have a good protocol how to do apoptosis measurements in adherent cells? thank you, Anke _________________________________________________________________ Get your FREE download of MSN Explorer at http://explorer.msn.com
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